Neurophotonics Journal 2025 November 2022 - October 2024

Development of fNIRS Biomarkers of Mental Workload

Demonstrating that short-channel regression significantly improves cortical activation estimates for working memory load assessment using functional near-infrared spectroscopy

Overview

Functional near-infrared spectroscopy (fNIRS) is a promising technology for monitoring cognitive workload in naturalistic settings. However, signals are contaminated by systemic physiological noise. This project establishes best practices for preprocessing fNIRS data to reliably detect cognitive load from cortical activation.

Working Memory

N-back paradigm across multiple difficulty levels

fNIRS Technology

Non-invasive optical brain imaging

Short-Channel Regression

Removing systemic noise from cortical signals

fNIRS Preprocessing Pipeline

Raw fNIRS Optical Density Motion Correction Short-Channel Regression Bandpass Filter HbO / HbR Concentration fNIRS Preprocessing Pipeline HbO HbR

Figure 1: Complete fNIRS preprocessing pipeline showing raw optical density signals, motion artifact correction, short-channel regression, and hemodynamic response extraction.

My Role

First Author
  • Experimental Design: Designed the N-back working memory paradigm with graded difficulty
  • Data Collection: Conducted fNIRS acquisition sessions with human participants
  • Signal Processing: Developed and compared preprocessing pipelines in MATLAB and Python
  • Statistical Analysis: Validated short-channel regression methodology with rigorous statistics
  • Supervision: Mentored student development of fnirsPy Python package

Methodology

N-back Task Paradigm A B C A D 2-back Match! Working Memory Load Levels: 0-back 1-back 2-back 3-back fNIRS Optode Placement Front Prefrontal Cortex Source Detector

Figure 2: (Left) N-back working memory task with 0-, 1-, 2-, and 3-back conditions. (Right) fNIRS optode configuration covering prefrontal cortex.

What's Innovative

This study provides systematic validation of short-channel regression for fNIRS-based cognitive load monitoring. Key findings:

+40% Effect Size Improvement
SC-Reg Best Practice
PFC Activation Locus

By using short-channel measurements to regress out scalp hemodynamics, we achieve more reliable cortical activation estimates, establishing best practices for the cognitive monitoring field.

Results

HbO Response Across N-back Levels Without SC-Regression 0-back 1-back 2-back 3-back ΔHbO (μM) With SC-Regression ✓ * ** 0-back 1-back 2-back 3-back +40% Effect Size * p < 0.05, ** p < 0.01

Figure 3: Comparison of oxygenated hemoglobin responses to increasing N-back load with and without short-channel regression, showing improved effect sizes after correction.

Publication

Functional near-infrared spectroscopy short-channel regression improves cortical activation estimates of working memory load

An, J., Schönhammer, J., Luft, A., et al.

Neurophotonics, 2025

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